A direct determination of a glucose-arsenic complex by electrospray ionization time of flight mass spectrometry.

Metallomics. 2011 Oct;3(10):1074-8.
Mandal SM.
Mass Spectrometry & Proteomics Lab. Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
Abstract - Electrospray ionization time of flight mass spectrometry (ESI-TOF-MS) was used to identify elemental ions from the glucose-arsenic interaction in the aqueous phase. In glucose solution, the most abundant ions were m/z 203, m/z 163, m/z 158, m/z 145 and m/z 115, whereas some additionalarsenic bearing ions, m/z 271, m/z 235 and m/z 213 were observed from a glucose-arsenic solution in alkaline pH (≥ 7.5) at 37 °C. The binding was best fitted to 1:1 isotherm model and the value of the dissociation constant (K(d)) was 39.8 μM. Results suggest that the polyatomic ions derived from glucose interact with the available arsenic ions in blood and form a complex which might play a role in diseases caused by arsenic exposure.